Preparation of Alkaline Peptone Water. To make about 50 bottles (i.e. 500 ml), you will need 5 g of peptone and 5 g of Sodium chloride. Dissolve the peptone and sodium chloride in the water. Adjust the reaction of the medium to pH 8.6 to 9.0 using 1 mol/L sodium hydroxide. Dispense the medium in 10 ml amounts in screw-cap bottles.

Buffered Peptone Water (BPW) is used as a pre¬enrichment medium for the isolation of Salmonella sp. from food products, particularly injured microorganisms. Available in the following size: G127-4.5 (225 mL), G127-22.5 (1.125L), G127-30 (1.5L), G127-68 (3.375L), G127-68-cs (80 units) Buffered Peptone Water is a pre-enrichment medium designed to help recovery of sub-lethally damaged Salmonellae before transfer to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. neutralizing buffered peptone water (nBPW) that incorporates neutralizing agents to reduce the effect of potential carryover of antimicrobial interventions. The additional neutralizing agents causethe buffer to appear cloudy. The new buffer is safe to use as a rinse (or swab) for poultry carcasses and parts, and it Peptone water is a buffered solution generally containing peptone—a soluble protein—which is then buffered to a neutral pH (approximately 7.0) using a base disodium phosphate. According to the FDA’s Bacteriological Analytical Manual (BAM) , it contains peptone, sodium chloride, two forms of phosphate (disodium and mono-potassium), and Buffered peptone water (BPW) is one of the most widely used pre-enrichment broths for Salmonella in a wide range of foods. Moreover, it is often the medium of choice in many published reference methods, including the current International Organization for Standardization (ISO) method (Anon. 1998), for the detection of Salmonella in foods.

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INTENDED USE Hardy Diagnostics Peptone Water, 0.1% is recommended as a diluent for microbial enumeration purposes as described by the Food and Drug Administration and The U.S. Pharmacopeia National Formulary (USP-NF) for use in pharmaceutical and cosmetic testing and by the American Public Health Association and Environmental Protection Agency for food, dairy and water industries. Buffered Peptone Water, Bag. A pre-enrichment medium for the isolation of salmonellae, particularly injured organisms, from foodstuffs & other sample types. Buffered Peptone Water acc. ISO 6579, ISO 19250, ISO 21528, ISO 22964, ISO 6887, FDA-BAM and EP GranuCult® - Find MSDS or SDS, a COA, data sheets and more information. 3M dehydrated media assist in keeping your lab practices. Convenient: flexibility of options, make what you need when you need it. Easy. Consistent.

2 g bacto peptone 0.25 g yeast extract 5.1 g Na 2 HPO 4 ∗7 H 2 O 4.2 g KH 2 PO 4; 20 g agar Autoclave 20 minutes. Reference: Fey et al.. LPB agar (buffered Lactose/Peptone, 1 liter) Stock Center Recipe. 1 g lactose 1 g bacto peptone

Created with Sketch. Toggle menu. 1-877-567-3598 in pH associated with the use of distilled water. This buffer is recommended for use in the United States Pharmacopeia (USP) for microbial limit testing of pharmaceutical products.1 The formulation is prepared according to current USP guidelines, which includes adjustment of the pH to 7.2 ± 0.1 prior to sterilization. Buffered Peptone Water, when used as a pre-enrichment, is recommended by the International Dairy Federation (IDF) and the International Commission on Microbiological Specifications for Foods for the isolation of Salmonella from food sources. 1 The high pH maintained over a 24- Autoclave at 121°C for 15 min. Final pH to 7.2±0.2. Note: commercial Modified Buffered Peptone Water (3M TM dehydrated media) can be substituted for the above formulation. Product Name or Identity: Buffered Peptone Water (Broth) Manufacturer’s Name: Acumedia Manufacturers, Inc. Emergency Phone No.: 517/372-9200 740 East Shiawassee Fax No.: 517/372-0108 Lansing, Michigan 48912 e-mail: foodsafety@neogen.com Date Prepared or Revised: May 2010 Chemtrec: (800) 424-9300 Outside US and Canada: (703) 527-3887